Environmental sustainability assessment of wooden furniture produced in Pakistan / Avaliação da sustentabilidade ambiental de móveis de madeira produzidos no Paquistão

Abstract Life cycle assessment was carried out for a conventional wooden furniture set produced in Mardan division of the Khyber Pakhtunkhwa province of Pakistan during 2018-19. Primary data regarding inputs and outputs were collected through questionnaire surveys from 100 conventional wooden furniture set manufacturers, 50 in district Mardan and 50 in district Swabi. In the present study, cradle-to-gate life cycle assessment approach was applied for a functional unit of one conventional wooden furniture set. Production weighted average data were modelled in the environmental impacts modelling software i.e., SimaPro v.8.5. The results showed that textile used in sofa set, wood preservative for polishing and preventing insects attack and petrol used in generator had the highest contribution to all the environmental impact categories evaluated. Total cumulative energy demand for wooden furniture set manufactured was 30,005 MJ with most of the energy acquired from non-renewable fossil fuel resources.

Resumo A abordagem de avaliação do ciclo de vida foi realizada para um conjunto de móveis de madeira convencional produzido na divisão Mardan da província de Khyber Pakhtunkhwa do Paquistão durante 2018-19. Os dados primários sobre entradas e saídas foram coletados por meio de pesquisas por questionário de 100 fabricantes de conjuntos de móveis de madeira convencionais, 50 no distrito de Mardan e 50 no distrito de Swabi. No presente estudo, a abordagem de avaliação do ciclo de vida do berço ao portão foi aplicada para uma unidade funcional de um conjunto de móveis de madeira convencional. Os dados da média ponderada da produção foram modelados no software de modelagem de impactos ambientais, isto é, SimaPro v.8.5. Os resultados mostraram que os têxteis usados ​​no conjunto de sofás, o preservativo de madeira para polir e prevenir o ataque de insetos e a gasolina usada no gerador tiveram a maior contribuição em todas as categorias de impacto ambiental avaliadas. A demanda total acumulada de energia para o conjunto de móveis de madeira fabricado foi de 30.005 MJ, com a maior parte da energia adquirida de recursos de combustíveis fósseis não renováveis.

Environmental sustainability assessment of wooden furniture produced in Pakistan

Abstract Life cycle assessment was carried out for a conventional wooden furniture set produced in Mardan division of the Khyber Pakhtunkhwa province of Pakistan during 2018-19. Primary data regarding inputs and outputs were collected through questionnaire surveys from 100 conventional wooden furniture set manufacturers, 50 in district Mardan and 50 in district Swabi. In the present study, cradle-to-gate life cycle assessment approach was applied for a functional unit of one conventional wooden furniture set. Production weighted average data were modelled in the environmental impacts modelling software i.e., SimaPro v.8.5. The results showed that textile used in sofa set, wood preservative for polishing and preventing insects attack and petrol used in generator had the highest contribution to all the environmental impact categories evaluated. Total cumulative energy demand for wooden furniture set manufactured was 30,005 MJ with most of the energy acquired from non-renewable fossil fuel resources.

Resumo A abordagem de avaliação do ciclo de vida foi realizada para um conjunto de móveis de madeira convencional produzido na divisão Mardan da província de Khyber Pakhtunkhwa do Paquistão durante 2018-19. Os dados primários sobre entradas e saídas foram coletados por meio de pesquisas por questionário de 100 fabricantes de conjuntos de móveis de madeira convencionais, 50 no distrito de Mardan e 50 no distrito de Swabi. No presente estudo, a abordagem de avaliação do ciclo de vida do berço ao portão foi aplicada para uma unidade funcional de um conjunto de móveis de madeira convencional. Os dados da média ponderada da produção foram modelados no software de modelagem de impactos ambientais, isto é, SimaPro v.8.5. Os resultados mostraram que os têxteis usados no conjunto de sofás, o preservativo de madeira para polir e prevenir o ataque de insetos e a gasolina usada no gerador tiveram a maior contribuição em todas as categorias de impacto ambiental avaliadas. A demanda total acumulada de energia para o conjunto de móveis de madeira fabricado foi de 30.005 MJ, com a maior parte da energia adquirida de recursos de combustíveis fósseis não renováveis.

Synovium and blood capillaries at the femoral attachment of anterior cruciate ligament are significantly abundant than those at the tibial attachment / Sinovial y capilares sanguíneos en la inserción femoral del ligamento cruzado anterior son significativamente más abundantes que los de la inserción tibial

SUMMARY: The anterior cruciate ligament (ACL) is a ligament that mainly controls the anterior and rotational mobility of the knee joint, and its surface is covered by a synovial membrane with large number of blood vessels. In general, nutritional supply to the ligament is from many capillaries in the adjacent synovium. However, statistical studies of the capillaries distributed to the ACL are insufficient. In this study, we examined cross-sectional histological images of the femoral attachment (femoral level), middle level of the tendon (middle level), and tibial attachment (tibial level) of the ACL and statistically analyzed blood capillary distribution among the three levels. The ACLs of 10 cadavers were divided into 5 equal sections, and 4mm-thick paraffin sections were made at the femoral level, middle level, and tibial level, and then hematoxylin-eosin (HE) staining were performed. The area of each transverse section was measured using Image-J 1.51n (U. S. National Institutes of Health, Bethesda, MD, USA). Fiber bundles of the ACL were relatively small and sparse in cross-sectional area at the femoral level and became larger and denser toward the tibial level. Many blood levels. The synovium at the attachment of ACL covered the surface of the fiber bundle and also penetrated deeply between the fiber bundles. In particular, the blood capillaries were densely distributed in the synovium at the femoral attachment rather than another two levels. Indeed, the number of capillaries were also most abundant in the femoral level. The cross-sectional ACL area at the femoral level is significantly small, however, the blood capillaries were most abundant. Therefore, when the ACL is injured, its reconstruction with preservation of the femoral ligamentous remnant may be clinically useful for remodeling of the grafted tendon.

El ligamento cruzado anterior (LCA) es un ligamento que controla principalmente la movilidad anterior y rotacional de la articulación de la rodilla, y su superficie está cubierta por una membrana sinovial con gran cantidad de vasos sanguíneos. En general, el suministro de nutrientes al ligamento proviene de muchos capilares en la sinovial adyacente. Sin embargo, los estudios estadísticos de los capilares distribuidos en el LCA son insuficientes. En este estudio, examinamos imágenes histológicas trans- versales de la inserción femoral (nivel femoral), el nivel medio del tendón (nivel medio) y la inserción tibial (nivel tibial) del LCA y analizamos estadísticamente la distribución de los capilares sanguíneos entre los tres niveles. Los LCA de 10 cadáveres se dividieron en 5 secciones iguales y se realizaron cortes en parafina de 4 µm de espesor a nivel femoral, medio y tibial, y luego se realizó tinción con hematoxilina-eosina (HE). El área de cada sección transversal se midió utilizando Image-J 1.51n (Institutos Nacionales de Salud de EE. UU., Bethesda, MD, EE. UU.). Los haces de fibras del LCA eran relativamente pequeños y escasos en el área de la sección transversal a nivel femoral y se hicieron más grandes y más densos hacia el nivel tibial. La membrana sinovial en la unión del LCA cubría la superficie del haz de fibras y también penetraba profundamente entre entre los haces de fibras. En particular, los capilares sanguíneos estaban densamente distribuidos en la unión femoral de la sinovial respecto a los otros dos niveles. De hecho, el número de capilares también fue más abundante a nivel femoral. El área transversal del LCA a nivel femoral era significativamente pequeña, sin embargo, los capilares sanguíneos fueron los más abundantes. Por lo tanto, cuando hay una lesión del LCA su reconstrucción con preservación del ligamento femoral remanente puede ser clínicamente útil para remodelar el tendón injertado.

Avaliação isocinética da força de contração excêntrica dos músculos isquiotibiais no período pré-operatório de pacientes com lesão de ligamento cruzado anterior / Isokinetic assessment of eccentric hamstring strength in the preoperative period of patients with anterior cruciate ligament injury

Introdução: A força de contração excêntrica é uma moderna medida para definir diferentes parâmetros no estudo da Ortopedia moderna, sendo considerada uns dos recentes temas mais discutidos no quesito ganho de força muscular. O objetivo do trabalho foi analisar o estado da musculatura flexora da coxa (isquiotibiais), através da avaliação de sua força de contração excêntrica, por meio do dinamômetro isocinético no período pré-operatório nos pacientes com lesão de ligamento cruzado anterior. Métodos: Estudo retrospectivo observacional entre agosto e dezembro de 2018 de uma clínica especializada em atendimento em Criciúma/SC. Resultados: A média do pico de torque excêntrico da musculatura posterior da coxa em flexão do membro afetado foi de 27,60 ± 10,56 kg, e do membro contralateral atingiu 27,47 ± 6,91 kg. O déficit de força entre os membros teve uma mediana de 9,14 % (0,0 - 61,45), e o tempo médio entre a lesão e o exame isocinético foi de 10,0 (1,0 - 48,0) meses. Observou-se que os maiores déficits de força foram encontrados naqueles indivíduos que tinham maior intervalo de tempo entre a lesão e a realização da avaliação isocinética. Conclusão: Nos indivíduos submetidos ao teste de dinamometria isocinética após lesão do ligamento cruzado anterior, evidenciou-se um déficit na força de contração excêntrica da musculatura isquiotibial entre o membro afetado e contralateral, o que ratifica achados da literatura a respeito da biomecânica articular do joelho. Além disso, maiores déficits foram encontrados naqueles pacientes com maior intervalo de tempo para realização do exame isocinético.

Introduction: Eccentric strength is a modern measurement for defining different parameters in the study of modern orthopaedics, being considered one of the most widely discussed topics in recent times within the subject of muscle strength gain. The aim of this work was to analyze the state of flexor muscles in the thigh (hamstring muscles) by evaluating their eccentric strength with an isokinetic dynamometer during the preoperative period of patients with anterior cruciate ligament injury. Methods: This is a retrospective observational study performed between August and December 2018 at a specialized clinic in Criciúma/SC. Results: The mean eccentric peak torque of the hamstring muscles during flexion of the affected limb was 27.60 ± 10.56 kg and that for the contralateral limb was 27.47 ± 6.91 kg. The strength deficit between limbs had a median value of 9.14% (0.0­61.45) and the mean time between the injury and isokinetic testing was 10.0 (1.0­48.0) months. Higher strength deficits were observed in individuals who had longer periods between the injury and isokinetic testing. Conclusion: In individuals who underwent isokinetic testing after an anterior cruciate ligament injury, there was a deficit between the eccentric hamstring strength of the affected limb and that of the contralateral limb, which corroborates findings in the literature regarding the biomechanics of the knee joint. Moreover, larger deficits were found in patients with longer periods between the injury and isokinetic testing.

Desempenho energético e pegada de carbono de um sistema de esgotamento sanitário centralizado no nordeste brasileiro / Energy performance and carbon footprint of a centralized sewage system in northeastern Brazil

RESUMO O objetivo do trabalho foi avaliar a operação e manutenção de um sistema de esgotamento sanitário centralizado, composto de três subsistemas, que atende 367 mil habitantes no Nordeste do Brasil. A avaliação do ciclo de vida considerou um inventário amplo de operação e manutenção do sistema de esgotamento sanitário com as redes de coleta, estações de tratamento de esgoto, disposição do esgoto tratado no corpo hídrico e gestão do lodo. O arranjo tecnológico das estações de tratamento de esgoto avaliadas incluiu o reator do tipo upflow anaerobic sludge blanket, seguido de lagoa aerada e lagoa de polimento em um subsistema, e upflow anaerobic sludge blanket seguido de reator de lodo ativado por aeração prolongada em dois subsistemas. O desempenho energético utilizou o método de demanda de energia acumulada e a pegada de carbono empregou o método de potencial de aquecimento global de 100 anos do Painel Intergovernamental sobre Mudanças Climáticas. O sistema de esgotamento sanitário avaliado demandou 5,12 MJ·m−3 e emitiu 4,08 kg CO2eq·m−3. As maiores contribuições do sistema de esgotamento sanitário avaliado foram a eletricidade, com 62% da demanda energética, e as emissões diretas para o ar, com 94% da pegada de carbono, sendo as emissões dos reatores upflow anaerobic sludge blanket com 76% da pegada de carbono. A identificação dos aspectos e impactos ambientais do sistema de esgotamento sanitário avaliado apoia a inovação tecnológica e gerencial para otimizar o desempenho energético e mitigar as emissões de gases de efeito estufa.

ABSTRACT This aim of this work was to evaluate the operation and maintenance of a centralized wastewater treatment system, composed of three subsystems, which serves 367 thousand inhabitants in northeastern Brazil. The life cycle assessment considered a comprehensive inventory of the wastewater treatment system operation and maintenance with the collection networks, wastewater treatment plants, disposal of the treated wastewater in the water body and sludge management. The technological arrangement of the evaluated wastewater treatment plants included the Upflow Anaerobic Sludge Blanket reactor, followed by aerated and polishing ponds in one subsystem, and Upflow Anaerobic Sludge Blanket followed by extended aeration activated sludge in two subsystems. The energy performance used the cumulative energy demand method and the carbon footprint used the global warming potential method for 100 years of the Intergovernmental Panel on Climate Change. The evaluated wastewater treatment system presented 5.12 MJ·m−3 and 4.08 kg CO2eq·m−3. The largest contribution of the evaluated wastewater treatment system was the electricity use with 62% of the energy demand and direct emissions to the air with 94% of the carbon footprint, being direct emissions from Upflow Anaerobic Sludge Blanket reactors with 76% of the carbon footprint. The identification of environmental aspects and impacts of the evaluated wastewater treatment system supports technological and management innovations to optimize its energy performance and mitigate greenhouse gases emission.

Reparación primaria con ancla bioabsorbible de ligamento cruzado anterior en avulsión tibial postraumática a dos años de seguimiento / Primary repair with bioabsorbable anchor of anterior cruciate ligament in post-traumatic tibial avulsion at 2 years of follow-up

Resumen: Introducción: Las lesiones del ligamento cruzado anterior son comunes; sin embargo, las avulsiones tibiales en adultos son raras. El estándar de oro continúa siendo la reconstrucción. En los últimos años se han reportado reparaciones primarias en lesiones de lado femoral mediante anclaje bioabsorbible. Caso clínico: Presentamos el caso de una reparación primaria de ligamento cruzado anterior en avulsión tibial en paciente masculino de 19 años posterior a sufrir de trauma directo con lesiones agregadas de ligamento cruzado posterior y esquina posterolateral. Se realizó reparación primaria por portal transtendón con sutura del ligamento cruzado anterior en cruz mediante FiberTape y FiberWire con anclaje SwiveLock 4.75 mm en huella tibial. Resultados: A los 24 meses de operado se encuentra con marcha independiente, arcos completos, con escalas de valoración clínica, Tegner antes de la lesión y posterior a la lesión en un nivel 6, escala de Lysholm 91, International Knee Documentation Committee (IKDC) subjetivo 73.6, IKDC objetivo en C y EQ-5D 0.79. Conclusión: Se puede reparar la avulsión de LCA en su lado tibial en ciertas condiciones con adecuada evolución y satisfacción para el paciente.

Abstract: Introduction: Anterior cruciate ligament injuries are common, however, tibial avulsions in adults are rare. The gold standard continues to be reconstruction. In recent years, primary repairs have been reported in femoral side lesions by bioabsorbable anchorage. Clinical case: We present the case of a primary repair of the anterior cruciate ligament in tibial avulsion in a patient 19 years old after presenting direct trauma with added injuries of the posterior cruciate ligament and posterolateral corner. Primary repair was performed by transtendon portal with suture of the anterior cruciate ligament with FiberTape and FiberWire and fixed with 4.75 mm SwiveLock anchor in tibial footprint. Results: At 24 months of surgery he is with independent gait, complete range of motion, with clinical assessment scales, Tegner before the injury and after the injury at a level 6, Lysholm scale 91, subjective International Knee Documentation Committee (IKDC) 73.6, objective IKDC in C and EQ-5D 0.79. Conclusion: The avulsion of ACL on its tibial side can be repaired in certain conditions with adequate evolution and satisfaction for the patient.

Effect of Licochalcone A on Apoptosis in Human Breast Cancer MDA-MB-231 Cells / 中国实验方剂学杂志

Objective:To investigate the effect of licochalcone A （LCA） on apoptosis in human breast cancer MDA-MB-231 cells， and to explore its possible mechanism. Method:MDA-MB-231 cells were treated with LCA of different concentrations， and<italic> </italic>cell counting kit-8 （CCK-8） assay was used to detect the cell viability. The cells were treated with LCA （10， 20， and 40 μmol·L^-1） for 24 h， and apoptosis was detected by Annexin V staining with fluorescein isothiocyanate （FITC） and propidium iodide （PI） （Annexin V-FITC/PI）. The level of intracellular reactive oxygen species （ROS） was detected by 2′，7′-dichlorodihydrofluorescein diacetate （DCFA-DA） fluorescent probe. Mitochondrial membrane potential （MMP） was detected by 5， 5′， 6， 6′-tetrachloro-1， 1′， 3， 3′-tetraethyl-imidacarbocyanine （JC-1） fluorescence probe. Western blot was used to detect the expression of cell apoptosis-related proteins， such as B-cell lymphoma-2 （Bcl-2） and Bcl-2-associated X protein （Bax）， and endoplasmic reticulum （ER） stress-related proteins， such as C/EBP homologous protein （CHOP）， activating transcription factor 4 （ATF4）， protein kinase R-like ER kinase （PERK）， p-PERK， eukaryotic translation initiation factor 2 alpha （eIF2<italic>α</italic>）， and p-eIF2<italic>α</italic>. Result:With the increase in the drug concentration （starting from 5 μmol·L^-1）， the cell viability decreased （<italic>P<</italic>0.05） with IC₅₀of 19.05 μmol·L^-1 as compared with the normal group. Additionally， the apoptosis rates of the LCA groups （10， 20， 40 μmol·L^-1） significantly increased （<italic>P</italic><0.05）， which reached 30.2% （<italic>P</italic><0.05） at LCA concentration of 40 μmol·L^-1. LCA （10， 20， and 40 μmol·L^-1） decreased the expression of Bcl-2 （<italic>P<</italic>0.05） and increased Bax expression （<italic>P<</italic>0.05） in a dose-dependent manner. Besides， the intracellular ROS level was elevated （<italic>P<</italic>0.05） and mitochondrial MMP was reduced （<italic>P<</italic>0.05） after LCA （10， 20， and 40 μmol·L^-1） treatment in a dose-dependent manner， leading to mitochondrial dysfunction. LCA （10， 20， and 40 μmol·L^-1） induced ER stress to up-regulate the expression of CHOP， ATF4， p-PERK， and p-eIF2<italic>α</italic> （<italic>P<</italic>0.05） in a dose-dependent manner. Conclusion:LCA can induce MDA-MB-231 cell apoptosis by increasing intracellular ROS level and reducing MMP to trigger mitochondrial dysfunction and ER stress.

Variabilidad en la ubicación de los túneles femoral y tibial en reconstrucción del ligamento cruzado anterior. Revisión narrativa / Variability in the positioning of femoral and tibial tunnels in the reconstruction of the anterior cruciate ligament: A NARRATIVE REVIEW

Introducción Aunque los estudios detallan el origen, la forma y la inserción del ligamento cruzado anterior (LCA), no hay consenso sobre la ubicación del punto adecuado para el posicionamiento del túnel femoral y tibial en la reconstrucción. El objetivo del estudio es revisar la literatura sobre la variabilidad en el posicionamiento de los túneles femoral y tibial en la reconstrucción del LCA. Materiales y Métodos Se realizó una búsqueda sistemática y revisión narrativa de la literatura publicada en las bases Medline, Embase, Central, Cochrane y Lilacs, usando términos MesH y libres. Se realizó una selección por título y resumen y revisión de textos completos. Se clasificaron las publicaciones por temas y se hizo una presentación narrativa de los hallazgos. Resultados Se incluyeron 25 estudios. Se encontró variabilidad en la selección de áreas de origen e inserción del LCA, tanto en modelos cadavéricos como pacientes: entre los reparos anatómicos para el posicionamiento del túnel femoral están la cresta intercondilea, cresta bifurcada, cresta del residente, ubicación según el sentido de las manecillas del reloj, remanente del LCA y para el túnel tibial el cuerno anterior del menisco lateral. También hubo variabilidad en los métodos intraquirúrgicos y ayudas imagenológicas como lo son el uso del portal artroscópico antero-medial, cuadrantes de Bernard, fluoroscopio y fluoroscopio navegado. Solo un estudio evaluó la variabilidad en el posicionamiento entre y dentro de cada cirujano mostrando relativa consistencia entre ellos. Discusión El sitio de posicionamiento del túnel femoral y tibial para la reconstrucción del LCA, queda determinado por la experiencia del cirujano al no tener guías objetivamente estandarizadas, para realizar la reconstrucción.

Background Although several studies have detailed the origin, shape, and insertion of the anterior cruciate ligament (ACL), there is still no consensus on the location of the appropriate point for the positioning of the femoral and tibial tunnels for its grafting. The purpose of this study is to review the literature on the variability in the positioning of femoral and tibial tunnels when a graft reconstruction of the ACL has to be performed. Methods A systematic search was carried out on the literature published in the Medline, Embase, Central, Cochrane, and Lilacs databases, using MesH and free terms. A selection was made by title and summary and a review of complete texts. The publications were classified by topic, and a narrative presentation of the findings was made. Results A total of 25 studies were included. Variability was found in the selection of areas of origin and insertion of the ACL in both cadaveric models and patients. Among the anatomical repairs for the positioning of the femoral tunnel are the intercondylar crest, bifurcated crest, the resident's crest, clockwise or counter-clockwise direction, remnant of the ACL, and for the tibial tunnel, the anterior horn of the lateral meniscus. There was also variability in the intra-surgical methods and imaging aids, such as the use of the antero-medial arthroscopic portal, Bernard's quadrants, fluoroscope, and navigated fluoroscope. Only one study evaluated the variability in positioning between and within each surgeon, showing relative consistency between them. Discussion The positioning site of tunnels for the reconstruction is determined by the experience of the surgeon, since there are no objectively standardised guides to perform the reconstruction.

Anti-inflammatory study on 9,9'-O-di-(E）-feruloyl-meso-5,5'-dimethoxysecoisolariciresinol (LCA), an active ingredient in Litsea cubeba (Lour.) Pers / 药学实践杂志

Objective To evaluate the anti-inflammatory effects of LCA, which is the active component from Litsea cubeba (Lour.) Pers. Methods Pharmacodynamic evaluations of ear swelling and cotton ball granuloma models were used in animal experiments. In vitro experiment, lipopolysaccharide (LPS) stimulated monocyte macrophage RAW264.7 cells were used to evaluate the anti-inflammatory activity of LCA by detecting the secretions of nitric oxide (NO), tumor necrosis factor-α (TNF-α), the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein. Results In ear swelling experiment, the extent of ear swelling was significantly lower in the LCA treated group than the model group. The inhibition rate was greater in the high LCA concentration group than the positive drug group. In addition, LCA reduced the weight of cotton ball granuloma markedly. At the cell level, LCA significantly inhibited the secretions of NO, TNF-α and reduced the expressions of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells. Conclusion LCA has significant anti-inflammatory effects in vitro and in vivo. The further studies are warranted for the development of anti-inflammatory drugs.

Precisión diagnóstica de la radiografía para la medición de túneles óseos en reconstrucción de ligamento cruzado anterior / X ray diagnostic accuracy for bone tunnel measurements in anterior cruciate ligament reconstruction

OBJETIVO: Determinar la precisión diagnóstica de las radiografías de rodilla para la medición de los diámetros de los túneles tibiales y femorales en reconstrucción de ligamento cruzado anterior (RLCA). MATERIALES Y PACIENTES: Estudio retrospectivo de precisión diagnóstica en pacientes con antecedentes de RLCA. Inclusión: RLCA realizada en el mismo centro, con el mismo equipo quirúrgico, entre 2011 y 2015; uso de cualquier tipo de injerto y de fijación; estudiados con radiografía (Rx) y tomografía computada (TC) durante postop en el mismo centro. Exclusión: desfase Rx-TC > 6 meses; cirugía entre estudios radiológicos. Análisis de túneles por 3 especialistas (OsiriX). La precisión diagnóstica consideró la TC como gold standard. Se midieron las diferencias inter e intra-observador, y las variables que interfirieron en las mediciones. RESULTADOS: 22 pacientes cumplieron los criterios de selección, determinando 528 mediciones en total (Rx y TC, fémur y tibia, 3 observadores, 2 tiempos). No hubo diferencias estadísticamente significativas en la medición de los túneles femorales (p » 0.8986), pero sí en cuanto a los diámetros tibiales (p » 0.0001). El análisis de precisión diagnóstica determinó una sobrevaloración de los diámetros óseos al usar la radiografía (10,5% en fémur, 10% en tibia). Hubo diferencias estadísticamente significativas inter-observador tanto en Rx como en TC (observador más joven con el resto); sin diferencias intra-observador. CONCLUSIÓN: La Rx como método diagnóstico del diámetro de túneles óseos luego de una RLCA sobreestima los valores reales, lo que empeora cuando el observador tiene menor experiencia.

OBJECTIVE: To determine the diagnostic accuracy of knee radiographs for the measurement of tibial and femoral tunnels diameters after an anterior cruciate ligament reconstruction (ACLR). MATERIALS AND PATIENTS: A retrospective study of the diagnostic accuracy in patients with a history of ACLR. Inclusion: surgery performed in the same center, same surgical team, between 2011 to 2015; any graft and fixation; studied with radiography (X-Ray) and computed tomography (CT) during postop in the same center. Exclusion: X-Ray-CT lag > 6 months; surgery between radiological studies. Tunnel analysis by 3 specialists (OsiriX). Diagnostic accuracy considered CT as a gold standard. The inter and intraobserver differences, and the variables that interfered in the measurements, were measured. RESULTS: 22 patients achieved the selection criteria, determining 528 measurements in total (X-Ray and CT, femur and tibia, 3 observers, 2 times). There were no statistically significant differences in the measurement of the femoral tunnels (p » 0.8986), but there were differences in the tibial diameters (p » 0.0001). Analysis of diagnostic accuracy determined an overestimation of the bone diameters when using the radiography (10.5% in the femur, 10% in the tibia). There were statistically significant inter-observer differences in both X-Ray and CT (younger observer with the rest); there were no intra-observer differences. CONCLUSION: X-Ray as a diagnostic method of the diameter of bone tunnels after an ACLR overestimates the real values, which worsens when the observer has less experience.

Progress in research on LCA2 gene therapy / 国际眼科杂志(Guoji Yanke Zazhi)

@#Leber's congenital amaurosis(LCA)is a genetic eye disease that can cause blindness. Infants with LCA may have a severe low vision or loss of vision at the early stage. The LCA2 type of this disease is related to RPE65 mutation. According to previous studies, there is no effective treatment for genetic retinal diseases including LCA2. In recent years, with the advances in gene therapy technology, great progress in the treatment of genetic retinal diseases has been made, among which the most successful one is the gene therapy of LCA2. This paper briefly introduces the development of the gene therapy of LCA2, and reviews the correlation between age and injection type, dosage, injection method, measuring method as well as therapeutic effect and the stability of therapeutic effect in previous clinic trials, which provides reference and clinical treatment experience for the clinical application of the gene therapy of LCA2 in China.

Ensayo pictórico: descripción del posicionamiento del túnel femoral en distintas técnicas de reconstrucción del ligamento cruzado anterior (lca) / Pictory essay: description of the positioning of the femoral tunnel in different techniques of reconstruction of the anterior cruciate ligament

En la práctica radiológica habitual el radiólogo se encuentra frecuentemente con distintas técnicas de reconstrucción del LCA que debe conocer de manera general para una adecuada descripción e interpretación de los hallazgos. En el presente ensayo pictórico exponemos diferentes tipos de técnica de reconstrucción del LCA, con énfasis en el concepto de reconstrucción anatómica, presentando distintas posiciones del túnel femoral, buscando entregar herramientas al radiólogo para reconocer la normalidad postoperatoria e interpretar posibles complicaciones.

In daily radiological practice, we frequently fid different ACL reconstruction techniques, which we must know in order to achieve adequate interpretation of the fidings. In this pictorial essay, we show different types of LCA reconstruction techniques, highlighting the anatomical reconstruction concept and presenting different positions of the femoral tunnel, seeking to deliver tools to the radiologist in order to recognize the normal postoperative fidings and possible complications.

Incidencia de artrofibrosis en reconstrucción primaria del ligamento cruzado anterior (LCA) con autoinjerto del tendón del cuádriceps / Incidence of arthrofibrosis in primary reconstruction of the anterior cruciate ligament (ACL) using quadriceps tendon graft

Introducción La artrofibrosis de rodilla es una complicación importante que se puede presentar en cirugía de reconstrucción primaria del ligamento cruzado anterior (LCA) y afecta negativamente a los resultados por pérdida de flexoextensión de la rodilla. El objetivo del estudio es establecer la existencia de artrofibrosis en reconstrucción primaria del LCA en pacientes en los cuales se utilizó técnica de autoinjerto de tendón del cuádriceps. Materiales y métodos Se realizó un estudio retrospectivo, una serie de casos, en que se incluyó a todos los pacientes con lesión del LCA a quienes se practicó cirugía durante un período de observación de 4 años, en los cuales se utilizó autoinjerto de tendón del cuádriceps sin taco óseo rotuliano. Resultados Se encontró a 127 pacientes con cirugía primaria del LCA con autoinjerto de tendón del cuádriceps sin bloqueo óseo rotuliano durante el período de observación. Cinco pacientes (3,9%) presentaron artrofibrosis que se clasificaron, de acuerdo con la clasificación de Shelbourne, como un paciente de tipo I, tres pacientes de tipo II y un paciente de tipo III. A todos los pacientes se les realizó artrólisis artroscópica total y un plan agresivo de rehabilitación. Todos recuperaron los rangos de movilidad y retornaron a su actividad deportiva. Discusión Mejoras en la técnica quirúrgica, en instrumental quirúrgico, en los protocolos de rehabilitación acelerada y en minimizar los factores de riegos asociados han disminuido considerablemente esta complicación. Nivel de evidencia clínica Nivel IV.

Background Knee arthrofibrosis is a significant complication that can occur in primary reconstruction surgery of the anterior cruciate ligament (ACL), and adversely affects the results due to loss of flexion-extension of the knee. The aim of this study is to establish the presence of arthrofibrosis in primary ACL reconstruction in patients in whom a quadriceps tendon autograft reconstruction technique was performed. Materials and methods A retrospective case series study was conducted over a four-year observation period on all patients with ACL lesion who were subjected to surgery, in which autograft of the tendon quadriceps without patellar bone block was used. Results A total of 127 patients underwent primary ACL surgery with quadriceps tendon autograft without patellar bone block during the observation period. The five (3.9%) patients that presented with arthrofibrosis were classified according to the Shelbourne classification as one type I patient, three type II patients, and one type III patient. All patients underwent total arthroscopic arthroscopy and aggressive rehabilitation. They all regained mobility ranges and returned to their sporting activity. Discussion Improvements in surgical technique, surgical instruments, accelerated rehabilitation protocols, and minimisation of associated risk factors have significantly reduced the incidence of arthrofibrosis. Evidence level Level IV.

Life Cycle Assessment (LCA) and Wind Power Generation: Application of the Methodology and the Generator to Power System

ABSTRACT Sustainability and economics are current issues in the generation of electricity. The energy matrix is diversified and some forms have stood out, such as wind. Some tools have been developed to improve project efficiency, one of which is Life Cycle Assessment (LCA). This presents solidity when assisting projects financially and environmentally, evaluating materials and processes. The present study aimed to analyze the application of LCA in a wind power plant. After searching the main databases and using filters, the analysis was developed from the information of an article that described the application of the LCA to a wind power plant using computational simulation, providing an overview of the production and potential environmental impacts for the process manufacturing, parts maintenance, disposal and recycling at end of lifespan. The phases, steps and the threshold diagram of the LCA system were observed. The results showed a high rate of reuse/recycling of some materials and significant reductions in emissions, financial costs and abatement of environmental payback if the materials were recycled at the end of lifespan. Recycling has shown positive environmental aspects and a significant cost reduction, allowing the expansion of markets and optimization of wind projects.

Quiste sinovial en la tibia luego de la reconstrucción del ligamento cruzado anterior: evaluación de 14 pacientes / Synovial cyst in the tibia after reconstruction of the anterior cruciate ligament: evaluation of 14 patients

Introducción: La aparición de quiste sinovial pretibial posterior a cirugía de reconstrucción de LCA es una complicaciónpoco frecuente, de origen multifactorial que puede suceder incluso años luego de la cirugía. El objetivo del presentetrabajo fue analizar una serie de pacientes tratados por esta complicación. Material y métodos: Se evaluaron retrospectivamente 14 pacientes operados de resección de quiste pretibial posterior auna cirugia de LCA entre el año 2008 y 2016. Se analizó el tipo de injerto, técnica quirúrgica e implante utilizado, la clínicadel paciente y el tiempo de aparición de los síntomas. Se realizó una evaluación radiológica pre y post operatoria. Sedescribió la cirugía de resección del quiste y la anatomía patológica. Evaluamos el índice de recidiva. Resultados: Nueve hombres y 5 mujeres con edad promedio de 38 años. El seguimiento promedio fue de 35 meses.Todos operados con isquiotibiales cuádruple y técnica trans-tibial. El tiempo promedio entre la cirugía de LCA y aparicióndel quiste fue de 29 meses. Clínicamente presentaban una tumoración entre 1.1 y 3 cm sin signos de inestabilidad. Através de Rx y RM se evidenció ensanchamiento del túnel tibial en todos los casos. Se rellenó el túnel tibial con injertoóseo en 7 casos. La anatomía patológica informó: 12 quistes sinoviales (5 asociados a remanentes de sutura) y 2 casosúnicamente fibrosis. Un paciente evolucionó con recidiva...

Introduction: Among complications following ACL reconstruction, the formation of a pre-tibial cyst in the site of the tibialtunnel is very rare and might happen even years after surgery. The purpose of this study was to analyze 14 patients with tibialsynovial cyst after ACL reconstruction.Material and method: We retrospectively evaluated patients operated between 2008 and 2016. We analyzed the graftselection and surgical technique for ACL reconstruction, the time between primary surgery and the onset of symptoms andthe clinical presentation. A pre and postoperative radiological evaluation was performed for every patient. Surgical techniquefor cyst excision, histological analysis and culture results were also analyzed. The recurrence rate was evaluated at finalfollow-upResults: Nine patients were male, with an average age of 38 years. The average follow-up was of 35 months. All ACLreconstructions were performed using hamstring graft and a trans-tibial technique. The average time between primary ACLsurgery and onset of the cyst was 29 months. All patients presented a palpable tumor at proximal tibia and a stable knee.The cyst size varied between 1.1 and 3 cm. In all cases, Rx and MRI could appreciate a widening of the tibial tunnel. Thetibial tunnels were filled with cancellous bone in 7 cases. Pathological anatomy reported 12 synovial cysts (5 associated withremaining suture) and 2 fibrosis. One recurrence was reported...

Relación entre la inclinación posterior de la tibia proximal y la inclinación meniscal. Estudio anatómico en resonancia magnética / The correlation between posterior tibial slope and meniscal slope. Anatomical study and magnetic resonance imaging

INTRODUCCIÓN: La geometría articular y las estructuras óseas se han mencionado como factores de riesgo para ruptura del ligamento cruzado anterior (LCA). La inclinación posterior de la tibia proximal (IPTP) es uno de los factores anatómicos mencionados. No se han encontrado estudios que reporten el rol del menisco externo en la estabilidad de la rodilla. El objetivo del siguiente estudio es determinar la relación entre la IPTP y la inclinación meniscal (IM) medidas por resonancia magnética (RM). MATERIAL Y MÉTODO: Se estudiaron 87 RM en 82 pacientes. Fueron excluidos pacientes con alteraciones anatómicas en la tibia proximal. Se realizaron las mediciones de la IPTP y la IM según el método de Hudeck. Se realizaron estudios de correlación para determinar la relación entre ambas variables. RESULTADOS: La edad media de los pacientes fue de 35.9 años. En el compartimento lateral la diferencia entre IPTP y la IM fue de 7.3° en promedio. El coeficiente Spearman entre IPTP del platillo externo y la IM es de 0.45. En el compartimento medial la diferencia entre la IPTP y la IM fue de 3.6°. El coeficiente Spearman entre la IPTP y la IM es de 0.57. CONCLUSIÓN: La correlación observada en ambos compartimentos es de baja a moderada lo que significa que la IPTP tiene poca participación en al valor final de la IM. Estos datos destacan la importancia del menisco externo en la biomecánica de la rodilla. Tipo de estudio: Serie de casos. Nivel de evidencia: IV.

BACKGROUND: Articular geometry and bony structures have been described as a risk factors for ACL ruptures. Posterior tibial slope´s one of the reported factors. We didn´t find studies describing the influence of the lateral meniscus in knee stability. Our aim is to determine the correlation between PTS and the meniscal slope (MS) in magnetic resonance images (MRI). METHODS: We studied 87 MRIs in 82 patients. Patients with anatomical changes in the proximal tibia (eg.: high tibial osteotomy) were discharged. The PTS and the MS was measured with the method of Hudeck. We did correlation tests to find any correlation between variables. RESULTS: Mean age was 35.9. In the lateral compartment the difference between PTS and MS was 7.3° and the Spearman tests was 0.45. In the medial compartment the difference between PTS and MS was 3.6° and the Spearmen tests was 0.57. CONCLUSIÓN: The correlation between both measures was low or moderate. The influence of the PTS in the MS it´s relative and we must consider the lateral meniscus in knee biomechanics. Type study: Number of cases. Level of evidence: IV.

Falla de injerto sintético en reconstrucción de ligamento cruzado anterior. Revisión en dos tiempos. Reporte de caso y detalles de técnica / Synthetic graft failure for ACL reconstruction. Revision surgery in two stages

Los injertos sintéticos representan una alternativa a los injertos biológicos para reconstrucción del ligamento cruzado anterior. Ciertas ventajas teóricas apoyan dicha técnica, la principal función del injerto sintético sigue siendo estructural. Las publicaciones respecto a esta alternativa quirúrgica brindan información controversial, y en la literatura no se observa evidencia suficiente aún que avale este sistema como alternativa en reconstrucción de LCA a largo plazo. En la literatura se describen múltiples complicaciones asociadas al injerto sintético. Se presenta un reporte de caso de falla de injerto sintético de LCA, el cual se resolvió con una planificación en dos tiempos quirúrgicos. Se detallan los hallazgos intraoperatorios y los detalles de técnica para dicha revisión quirúrgica.

Synthetic grafts represent an alternative to biological grafts for anterior cruciate ligament reconstruction. Certain theoretical advantages support this technique; the main function of the synthetic graft remains structural. Publications provide controversial information regarding this surgical alternative, and there is not enough data to support this system as an alternative to long-term outcomes ACL reconstruction. Multiple complications associated with synthetic graft are described in the literature. We present a case report of synthetic ACL graft failure, resolved in two stages ACL revision surgery. We describe intraoperative findings and technical details.

Benéficos da hidroterapia na lesão de ligamento cruzado anterior / Beneficial for hydrotherapy in anterior cruciate ligament injury

Introdução: O joelho é uma articulação sucessível a lesões devido a suas características anatômicas e composição que não lhe oferece uma segurança, pois não está protegido por tecido adiposo e tecido muscular. Entre as diversas lesões comuns, está a lesão do ligamento cruzado anterior (LCA) bastante comum entre atletas [1]. A hidroterapia é um recurso fisioterapêutico que proporciona ao paciente benefício terapêutico através das propriedades físicas e do aquecimento da água. Promove rápido ganho de amplitude e de movimento (ADM) e diminuição do edema [2]. Objetivo: Mostrar os benefícios da hidroterapia na reabilitação de lesões de LCA. Métodos: A metodologia aplicada compõe-se através de uma pesquisa do tipo revisão bibliográfica. Foram consultadas bases de dados eletrônicas: Scielo e EBSCO, no idioma português dos últimos 10 anos. Resultados: A hidroterapia tem uma atuação efetiva no pós-operatório do LCA, existe uma menor intensidade álgica que se dá através da temperatura da água que é de aproximadamente 33º graus Celsius, produzindo relaxamento muscular. Como não existe ação gravitacional o paciente conseue adquirir uma melhora da marcha, diminuindo o impacto causado na articulação, assim vencendo a apreensão e dando mais liberdade e independência ao paciente [3]. Comparando o tratamento hidrocinesioterápico com a cinesioterápico pode-se verificar que o tratamento de fisioterapia aquática trouxe benefícios em ganho de ADM, manutenção do trofísmo muscular, ganho de equilíbrio da relação agonista/antagonista, só teve um ponto que não foi positivo, pois não foi possível recuperar por completo a força muscular extensora que se justifico pela ação da gravidade dentro da água [4]. Em um estudo realizado por Belchior [5] entendeu-se que os exercícios aquáticos promovem um rápido retorno de atividades atléticas, o que é recomendado na fase inicial de um programa de reabilitação, com tudo se deve associar a fisioterapia convencional ao tratamento para que tenha um efeito significativo para o paciente que fez uma reconstrução do LCA. Conclusão: Conclui-se que a hidroterapia é um excelente recurso para a reabilitação no pósoperatório de LCA, promove uma menor intensidade álgica, obtém ganho de força e tônus muscular, aumento da ADM e proporciona o retorno precoce de pacientes a suas funções diárias, e no caso de atletas, uma volta mais rápida e efetiva ao esporte. (AU)

Tratamiento de la lesión del ligamento cruzado anterior en pacientes con fisis abiertas / Treatment of anterior cruciate ligament injury in patients with open physis

Contexto: las roturas del ligamento cruzado anterior (LCA) en pacientes con fisis abiertas representan el 3,3% de las roturas del LCA, En el tratamiento de las roturas intersticiales del LCA en pacientes con fisis abiertas existe controversia en la elección entre tratamiento conservador y tratamiento quirúrgico. Objetivo: analizar la reconstrucción del ligamento cruzado anterior LCA con fisis abierta en el Servicio de Ortopedia y Traumatología del Hospital de Especialidades FFAA No 1. Sujetos y métodos: estudio retrospectivo en pacientes sometidos a cirugía artroscópica para tratamiento de lesión del LCA con fisis abiertas, en el periodo enero 2012 y abril de 2015. En relación al tratamiento, se utilizó la técnica transfisaria completa (monotúnel); el injerto se fija al fémur con un endobotón y a la tibia con un tornillo de interferencia; el injerto fue obtenido del músculo semitendinoso. Resultados: fueron intervenidos 9 pacientes de sexo masculino, con edades entre 13 y 16 años, con lesiones del ligamento cruzado anterior en rodilla derecha (n=7, 78%) e izquierda (n=2, 22%); la ruptura del menisco es la lesión asociada más frecuente. Conclusión: las roturas del LCA con fisis abiertas son cada vez más frecuentes en la actualidad. La reconstrucción del LCA debe ser precoz para evitar lesiones condrales y meniscales. Se recomienda el tratamiento quirúrgico por los óptimos resultados clínicos y funcionales. (AU)

Background: anterior cruciate ligament (ACL) tears in open physis patients represent 3.3% of ACL tears. In the treatment of ACL interstitial tears in patients with open physis, there is controversy in the choice between conservative treatment and surgical treatment. Objective: to analyze the reconstruction of the anterior cruciate ligament ACL with open physis in the Orthopedics and Traumatology Service of the Specialty Hospital FFAA No 1. Subjects and methods: retrospective study in patients undergoing arthroscopic surgery for treatment of ACL injury with open physis, in the period January 2012 and April 2015. In relation to the treatment, the complete transfusion technique (monotunnel) was used; the graft is fixed to the femur with an endobotton and to the tibia with an interference screw; the graft was obtained from the semitendinous muscle. Results: were operated on 9 male patients, with ages between 13 and 16 years, with injuries of the anterior cruciate ligament in the right knee (n = 7, 78%) and left (n = 2, 22%); the rupture of the meniscus is the most common associated lesion. Conclusion: ACL tears with open physis are becoming more frequent today. The reconstruction of the ACL must be early to avoid chondral and meniscal lesions. Surgical treatment is recommended for optimal clinical and functional results. (AU)

Estudio comparativo de reconstrucción de ligamento cruzado anterior con y sin lesiones condrales; resultados funcionales a largo plazo / Anterior cruciate ligament reconstruction with and without chondral lesions; long term functional results

INTRODUCCIÓN: La lesión del ligamento cruzado anterior (LCA), es una patología frecuente, y el éxito de la reconstrucción depende de diversos factores, tales como la técnica quirúrgica empleada, la edad del paciente, índice masa corporal, nivel de actividad y lesiones asociadas. El objetivo de este estudio es comparar los resultados funcionales a largo plazo de pacientes con lesiones condrales tratadas y sin lesiones condrales sometidos a la reconstrucción de LCA (RLCA), con autoinjerto semitendinoso-gracilis (ST-G). MATERIALES Y MÉTODOS: Estudio analítico retrospectivo. Cuarenta y nueve pacientes (34 hombres y 15 mujeres), fueron sometidos a la RLCA con autoinjerto ST-G. Se compararon dos grupos. Grupo RLCA + LC: 24 pacientes con lesiones condrales ICRS grado III y IV tratadas en el mismo acto quirúrgico y grupo RLCA: 25 pacientes sin lesiones condrales concomitantes. Las edades promedio fueron de 32 y 28 años en cada grupo respectivamente. La funcionalidad de los pacientes a largo plazo fue evaluada con el test de Lysholm, IKDC y retorno deportivo. Se compararon los promedios de los scores funcionales según cada grupo con Test de Mann-Whitney. La asociación entre ambos grupos y el retorno deportivo se analizó con Test de Chi2. Los análisis estadísticos se realizaron con Stata IC 13. RESULTADOS: El seguimiento promedio fue de 106,8 meses (rango: 86­125). Los resultados funcionales obtenidos al comparar el grupo RLCA + LC vs grupo RLCA fueron significativamente peores en el primero, con un promedio del score de Lysholm de 84,87 vs 94,52 (p = 0.0001) e IKDC de 84,43 vs 92,08 (p = 0,004), respectivamente. El retorno deportivo de cada grupo fue de 70,8% y 88% (p = 0,136). CONCLUSIÓN: La funcionalidad a largo plazo de los pacientes con una RLCA y que tengan alguna LC profunda tratada concomitante, es significativamente inferior que aquellos pacientes con RLCA aislada. No existen diferencias significativas en el porcentaje de retorno deportivo entre ambos grupos.

INTRODUCTION: Anterior cruciate ligament injury is a frequent sports injury, and successful reconstruction depends on diverse factors, such as surgical technique, age, body mass index, level of activity and other concomitant cartilage and meniscal lesions. The objective of this study is to retrospectively compare the long term clinical outcomes of ACL hamstring tendon autograft reconstruction with and without concomitant cartilage injuries. MATERIALS AND METHODS: Forty nine patients (34 men and 15 women) underwent single bundle transtibial ACL reconstruction and were divided in two groups (Group 1: 24 patients with chondral lesions grade III and IV of the ICRS, and Group 2: 25 patients without chondral lesions). Mean age of both groups was 32 and 28 years, respectively. Clinical outcome was evaluated with Lysholm test, IKDC and return to previous level of sport activities. Mean values of of funcional outcome scores between both groups were compared with Mann-Whitney test. Return to previous level of activity in both groups was analyzed with Chi-2 test. The statistical analysis was performed with Stata IC 13. RESULTS: The mean follow-up period was 106.8 months (range: 86­125). Mean Lysholm test for group 1 vs group 2 was 84.87 and 94.52 (p < 0.001), mean IKDC was 84.43 and 92.08 (p < 0.001), and return to previous sports was 70.8% vs 88% (p = 0.136) for each group. CONCLUSION: Long term clinical outcomes in patients with chondral lesions that underwent ACL reconstruction were significantly lower than patient with isolated ACL injuries. No statistical differences were found in the return to previous sport activities.